Publications:
1. Research Publications in Refereed Journals.
1. Inoué, S., G.G. Borisy and D.P. Kiehart. 1974. Growth and lability of Chaetopterus oocyte mitotic spindles isolated in the presence of porcine brain tubulin. J. Cell Biol. 62: 175-184.
2. Tilney, L.G., D.P. Kiehart, C.H. Sardet and M. Tilney. 1977. The polymerization of actin. IV: The role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm. J. Cell Biol. 77: 536-550.
3. Kiehart, D.P. 1981. Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium sequestering system. J. Cell Biol. 88: 604-617.
4. Kiehart, D.P., I. Mabuchi and S. Inoué. 1982. Evidence that myosin does not contribute to force production in chromosome movement. J. Cell Biol. 94: 165-178.
5. Eisen, A., D.P. Kiehart, S.J. Wieland and G.T. Reynolds. 1984. Temporal sequence and spatial distribution of early events of fertilization in single sea urchin eggs. J. Cell Biol. 99: 1647-1654.
6. Kiehart, D.P. and T.D. Pollard. 1984a. Inhibition of Acanthamoeba actomyosin-II ATPase activity and mechanochemical function by specific monoclonal antibodies. J. Cell Biol. 99: 1024-1033
7. Kiehart, D.P. and T.D. Pollard. 1984b. Stimulation of Acanthamoeba actomyosin ATPase activity by myosin-II polymerization. Nature 308: 864-866.
8. Kiehart, D.P., D.A. Kaiser, and T.D. Pollard. 1984a. Monoclonal antibodies demonstrate limited structural homology between myosin isozymes from Acanthamoeba. J. Cell Biol. 99: 1002-1014.
9. Kiehart, D.P., D.A. Kaiser, and T.D. Pollard. 1984b. Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of filament formation by antibodies that bind to specific sites on the myosin-II tail. J. Cell Biol. 99: 1015-1023.
10. Wong, A.J., D.P. Kiehart and T.D. Pollard. 1984. Myosin from human erythrocytes. J. Biol. Chem. 260: 46-49.
11. Hagen, S.J., D.P. Kiehart, D.A. Kaiser, and T.D. Pollard. 1986. Characterization of monoclonal antibodies to Acanthamoeba myosin-I that cross-react with both myosin-II and low molecular mass nuclear proteins. J. Cell Biol. 103: 2121-2128.
12. Kiehart, D.P. and R. Feghali. 1986. Cytoplasmic myosin from Drosophila. J. Cell Biol. 103: 1517-1525.
13. Dubreuil, R., T.J. Byers, D. Branton, L.S.B. Goldstein and D.P. Kiehart. 1987. Drosophila spectrin. I. Characterization of the purified protein. J. Cell Biol. 105: 2095-2102.
14. Byers, T.J., R. Dubreuil, D. Branton, D.P. Kiehart and L.S.B. Goldstein. 1987. Drosophila spectrin. II. Conserved features are revealed by analysis of cDNA clones and fusion proteins. J. Cell Biol. 105: 2103-2110.
15. Kiehart, D.P., M.S. Lutz, D. Chan, A.S. Ketchum, R. Laymon, B. Nguyen, and L.S.B. Goldstein. 1989. Identification of the gene for fly non-muscle myosin heavy chain: Drosophila myosin heavy chains are encoded by a gene family. EMBO J 8: 913-922.
16. Pesacreta, T., T.J. Byers, R.R. Dubreuil, D.P. Kiehart, and D. Branton. 1989. Localization of spectrin and actin during development of the Drosophila embryo. J. Cell Biol. 108: 1697-1709.
17. Dubreuil, R., C.T. Stewart, T.J. Byers, and D.P. Kiehart. 1990. A ß-spectrin isoform from Drosophila (ßH) is similar in size to vertebrate dystrophin. J. Cell Biol. 111: 1849-1858.
18. Ketchum, A.S., C.T. Stewart, M. Stewart and D.P. Kiehart. 1990. The complete sequence of the nonmuscle myosin heavy chain transcript: Conserved sequences in the myosin tail and differential splicing in the 5' untranslated sequence. Proc. Natl. Acad. Sci. USA 87: 6316-6320.
19. Rimm, D.L., D.A. Kaiser, D. Bhandari, P. Maupin, D.P. Kiehart, and T.D. Pollard. 1990. Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution mapping and characterization of monoclonal antibody. J. Cell Biol. 111: 2405-2416.
20. Young, P., T. Pesacreta and D.P. Kiehart. 1991. Dynamics of cytoplasmic myosin distribution during early Drosophila development. Development 111:1-14.
21. Karess, R.E., Chang, X.-j., K.A. Edwards, S. Kulkarni, I Aguilera and D.P. Kiehart. 1991. The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila. Cell 65:1177-1189.
22. Young, P.E., A.M. Richman, A.S. Ketchum and D.P. Kiehart. 1993.Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. Genes & Devel. 7:29-41. (see also Comment on this work by M. Titus. 1993. Multitasking with Myosin. Trends in Genetics 9:187-188).
23. Edwards, K. A., R. A. Montague, S. Shepard, B. A. Edgar, R. L. Erikson and D. P. Kiehart. 1994. Identification of Drosophila cytoskeletal proteins by induction of abnormal cell shape in fission yeast. Proc. Natl. Acad. Sci. USA 91:489-493. (this paper was highlighted in the “Monitor” section of Trends in Genetics as a recent paper of interest in the primary journals; 1994 Trends in Genetics 10:309).
24. Thomas, G. H. and D. P. Kiehart. 1994. ßHeavy-spectrin has a restricted tissue and subcellular distribution during Drosophila embryogenesis. Development 120:2039-2050.
25. Edwards, K. A., X.J. Chang, and D.P. Kiehart. 1995 Essential light chain of Drosophila nonmuscle myosin II. J. Muscle Res. and Cell Motility 16:491-498.
26. Mansfield, S.G., D.Y. Al-Shirawi, A.S. Ketchum, E.Clarie Newbern and D.P. Kiehart. 1996. Molecular organization and alternative splicing in zipper, the gene that encodes the Drosophila nonmuscle myosin II heavy Chain. J. Mol. Biol. 255:98-109.
27. Edwards, K.A. and D.P. Kiehart. 1996. Drosophila Nonmuscle myosin II has multiple essential roles in imaginal disc and egg chamber morphogenesis. Development 122:1499-1511.
28. Pederson, J.D., D.P. Kiehart, and J.W. Mahaffey. 1996. The role of HOM-C genes in segmental transformations: reexamination of the Drosophila Sex combs reduced embryonic phenotype. Dev. Biol. 180:131-142.
29. Edwards, K.A., M. Demsky, R.A. Montague, N. Weymouth & D.P. Kiehart. 1997. GFP-Moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Devel. Biol. 103-117. (see also newspaper article about this work by Robert Cooke “They’re Not Flying Blind Anymore”, Newsday, Tuesday, 11 November 1997).
30. Thomas, G.H., E. Claire Newbern, C.C. Korte, M.A. Bales, S.V. Muse, A.G. Clark, and D.P. Kiehart. 1997. Intragenic Duplication and Divergence in the Spectrin Superfamily of Proteins. Mol. Biol. Evol. 14:1285-1295.
31. Halsell, S.R. & D.P. Kiehart. 1998. Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis. Genetics 148: 1845-1863.
32. Thomas, G. H., D.C. Zarnescu, A.E. Juedes, M.A. Bales, A. Londergan, C.C. Korte and D.P. Kiehart. 1998. Drosophila ßH-spectrin is essential for development and contributes to specific cell fates in the eye. Development 125:2125-2134.
33. Aitken, P.G., A.J. Borgdorff, A.J.A. Juta, D.P. Kiehart, G.G. Somjen & W.J. Wadman. 1998. Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons. Pflugers Arch. – Eur. J. Physiol. 436:991-998.
34. Crawford, J.M., N. Harden, T. Leungi, L. Lim and D. P. Kiehart. 1998. Cellularization in Drosophila melanogaster is disrupted by the inhibition of Rho activity and the activation of Cdc42 function. Devel. Biol. 204:151-164.
35. Ohashi, T., D.P. Kiehart and H.P. Erickson. 1999. Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin–green fluorescent protein. Proc. Natl. Acad. Sci. USA. 96:2153-2158. (see also Commentary on this work by R. Hines. 1999. The dynamic dialogue between cells and matricies: Implications of fibronectin’s elasticity. Proc. Natl. Acad. Sci. USA. 96: 2588-2590.). (see commentary) (see movie)
36. Kiehart, D.P., C.G.Galbraith, K.A. Edwards, W.L. Rickoll and R.A. Montague. Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila. 2000. J. Cell Biol. 149:471-490. (See also Dove, A.W. 2000 In Brief “Cell Sheet Morphogenesis in Drosophila” J. Cell Biol. 149: 1-2).(see sGMCA sequence)
37. Champagne, M.B., K.A. Edwards, H.P. Erickson and D.P. Kiehart. 2000. Drosophila stretchin-MLCK is a novel member of the titin/myosin light chain kinase family. J. Molec. Biol. 300:759-777.
38. Halsell, S.R., B.I. Chu and D.P. Kiehart. 2000. Genetic analysis demonstrates a direct link between Rho signaling and nonmuscle myosin function during Drosophila morphogenesis. Genetics 155:1253-1265.
39. Crawford, J.M., Z.Su, O. Varlamova, A.R. Bresnick and D.P.Kiehart. In Press. Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization. European J. Cell Biology.
40. Su, Z. and D.P.Kiehart. In Press. Protein kinase C phosphorylates nonmuscle myosin-II from Drosophila but regulation of myosin function by this enzyme is not required for viability in flies. Biochemistry.
41. J.W. Bloor and D. P. Kiehart. (2001) zipper nonmuscle myosin-II functions downstream of PS2 integrin in Drosophila myogenesis and is necessary for myofibril formation. Developmental Biology 239, 215-228.