Stable C-13 Analysis and Carbon Dioxide

Introduction

Carbon, like most biologically significant elements is found in several different isotopic forms. An isotope is an element which varies in the number of neutrons in the nucleus. Carbon with 12 neutrons (12C) is the dominant isotope found in 99.89% abundance compared with the other stable carbon isotope, 13C which is found in 1.11% according to the Pee Dee Belemnite (PDB) standards.

Click on the part of the picture you are interested in.

These stable isotopes are important in the study of ecological systems over time because the different isotopes of carbon (or nitrogen, oxygen, and hydrogen) are used in different abundances in living things. The ratios of 12C/13C absorbed and released both into the atmosphere and in various tissues by plants, animals, and microorganisms are different from the standard abundances. For example most plants do not fix 13C as readily as they do 12C. Thus the 13C signature of the air changes as plants fix more 12C then 13C. By measuring changes in the 12C/13C signiture of various plant and animal tissues, the atmosphere, and at different places in the canopy much can be learned about biological/physical properties of life.

This website looks into the physiological ecology of plant's carbon dioxide use by tracing the tool of 13C stable isotope analysis through the current available literature. Included are 13C analysis of various ecologically pertinent scale levels namely soil, leaves, canopies, and the atmosphere. Some topics touched upon are are:
--soil organic carbon composition
--plant water use efficiency
--changes in the atmospheric carbon dioxide concentration
--gas exchange within the canopy


So click your way through the different bio-scales and learn about this high-tech and highly useful tool.

 

 

This page was put together as a class project for Phisiological Plant Ecolgy taught by Rob Jackson at Duke University for more pages on various topics in physiological plant ecology go to: bio265.

Home Atmosphere Level Canopy Level Leaf Level Soil Level Mass spec References

Page maintained by Sheela Agarwal and Meghan O'Donoghue
Last modified 11/24/02

link to ppt