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Bio
103 Announcements!!!
Please
note that Bio103L lecture starts Monday, 25 August and is
in the French Sci. 2231.
Labs
start Monday, 1 September at regularly scheduled times (see
below).
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Title:
General Microbiology
Department: Biology
Course number: Bio
103L
Semester: Fall 2008
Instructor: Dr. Rytas Vilgalys, fungi@duke.edu
Office: Bio. Sci. 346
Office hours: TBA
Lab Administrator:
Dr. Heath O'Brien, heo3@duke.edu
Lecture:
Mon& Wed, 8:45 - 9:35
French Sci. 2231
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Lab
sections:
Section 1 (MW, 10:05 - 12:05, 0045 Bio Sci)
Section 2 (MW, 10:05 - 12:05, 0039 Bio Sci)
Section 3 (MW, 1:15 - 3:15, 0045 Bio Sci)
Section 4 (MW, 2:50 - 4:50, 0039 Bio Sci)
Section 5 (TuTh, 9:15 - 11:15, 0045 Bio Sci)
Section 6 (TuTh, 10:05 - 12:05, 0039 Bio Sci)
Section 7 (TuTh, 1:15 - 1:15, 0045 Bio Sci)
Section 8 (TuTh, 2:50 - 4:50, 0039 Bio Sci) |
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| Prerequisites:
One course in a biological science. |
| Course
description: The course consists of a lecture and
laboratory, each of which meets twice a week. Lectures include
an overview of the history of microbiology, and cover both classical
and modern bacteriological principles. Topics discussed include
prokaryotic and eukaryotic structure, criteria used in classification,
physiology (including reproduction, growth and metabolism),
genetics (classical and molecular), infection and immunity,
and environmental and industrial aspects
involving microorganisms.
The laboratory
exercises allow students to demonstrate many of the principles
discussed and provide primary training in basic bacteriological
skills. The techniques used during the course include pure
culture methods, staining methods, microscopy, enzyme assays,
serological and immunological procedures, antibiotic testing,
molecular cloning, and electrophoresis of nucleic acids. Emphasis
is placed upon demonstration of the student's skills and on
analysis of experimental results. During the last several
weeks of the semester, students use the techniques learned
to identify two unknowns.
Warning:
Many of the species we use in Bio103L (approximately 50%)
are considered either opportunistic pathogens or pathogenic.
This means that, even though they may occur in or on our bodies
and are members of our normal microflora, given the right
circumstances (e.g., a compromised immune system, a
point of entry to the body such as cuts or scrapes, or post-surgery
conditions), these microorganisms may abnormally spread, multiply,
and cause disease. A compromised immune system does not just
mean HIV, but includes periods when your body is dealing with
the common cold, changes in diet, stress, or chemotherapy.
If you have any reason to be concerned about taking this course,
please talk with your physician prior to enrolling in BIo103L
and participating in the laboratory procedures.
If you do not have
a compromised immune system and are concerned for minor reasons
(such as cuts and scrapes), be cautious and wear gloves in
lab. No one will fault you for being careful. Even benign
microbes may evolve pathogenicity, so treat all microorganisms
as potential pathogens and follow lab safety protocols. The
microbiology staff does!
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| Text
and laboratory manual: (available at the Duke University
Bookstore)
Brock
Biology of Microorganisms. Madigan, M., J. Martinko, P. Dunlap and D. Clark. 2008. 12th edition. Pearson Education, Inc.,
Upper Saddle River, NJ.
Laboratory
Exercises for Bio 103 General Microbiology. O'Brien, H. and R. Vilgalys. 2008. Bio103L.
Duke University, Department of Biology, Durham, NC.
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| Exams:
There are two lecture exams and a final. Quizzes are given regularly
during lab, and there is a lab practical at the end of the semester.
Exam
format: Exam questions will be multiple choice
and short answer, along with short essays. The extent of the
material covered on exams will be announced in class. The
syllabus shown is tentative and meant only as a guide. However,
the exam dates are firm. Students are expected to conform
to the Judicial Code and Honor Commitment regarding exams.
Make-up
exam policy: Make-ups will usually be given within
one week after the scheduled exam, at a time and place to
be announced. Make-ups for reasons other than illness require
notification in advance. Please do not call the instructor
directly before an exam. It is your responsibility to insure
that each exam is taken at the proper time.
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| Grade
based on:
Lecture:
69% of total grade
| Exam 1 .....................................
| 23%
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| Exam 2 .....................................
| 23%
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| Final...........................................
| 23%
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Lab:
31% of total grade
| Quizzes & Homework...................................
| 25% |
| Topical Assignment......................................
| 10% |
| Laboratory Report.........................................
| 15% |
| Unknown Report...........................................
| 20% |
| Lab Final.........................................................
| 20% |
| Attendance & Participation*........................
| 10% |
| Total...............................................................
| 100% |
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