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Bio 103 Announcements!!!
Welcome BIo 103 -- General Microbiology! Please see our course content on Blackboard!
We have moved the course website to blackboard. Guest access is also allowed on our course site!

Still considering taking Microbiology? -- Students planning on enrolling in this course should note that this course requires students to work extra lab hours later in the semester, in addition to the regularly scheduled lab sections. If you enroll in this course, you will need to come during extra hours, possibly every 24-48 hrs. Example schedules of extra hours for past labs are available on Blackboard.
Title: General Microbiology
Department: Biology
Course number: Bio 103L
Instructor: Changes depending on Semester
Lab Administrator:
(Until July 14th, 2006) Dr. Annette M. Golonka, amg9@duke.edu
(After July 14th, 2006) Dr. Heath O'Brien, heo@duke.edu
Lecture: Monday, Wednesday, & Friday, 11:55 AM - 12:45 PM
Location may differ each semester -- check schedule.

Lab sections: Biological Sciences, room 0045 (new teaching facility in subbasement)
Sect.1: Mon & Wed AM, 9:15 - 11:15 AM
Sect. 2: Mon & Wed PM, 1:15 - 3:15 PM
Sect. 3: Tues & Thurs AM, 10:05 AM - 12:05 PM
Sect. 4: Tues& Thurs PM, 2:50 - 4:50 PM

Prerequisites: One course in a biological science.
Course description: The course consists of a lecture and laboratory, each of which meets twice a week. Lectures include an overview of the history of microbiology, and cover both classical and modern bacteriological principles. Topics discussed include prokaryotic and eukaryotic structure, criteria used in classification, physiology (including reproduction, growth and metabolism), genetics (classical and molecular), infection and immunity, and environmental and industrial aspects involving microorganisms.
The laboratory exercises allow students to demonstrate many of the principles discussed and provide primary training in basic bacteriological skills. The techniques used during the course include pure culture methods, staining methods, microscopy, enzyme assays, serological and immunological procedures, antibiotic testing, molecular cloning, and electrophoresis of nucleic acids. Emphasis is placed upon demonstration of the student's skills and on analysis of experimental results. During the last several weeks of the semester, students use the techniques learned to identify two unknowns. 

Warning: Many of the species we use in Bio103L (approximately 50%) are considered either opportunistic pathogens or pathogenic. This means that, even though they may occur in or on our bodies and are members of our normal microflora, given the right circumstances (e.g., a compromised immune system, a point of entry to the body such as cuts or scrapes, or post-surgery conditions), these microorganisms may abnormally spread, multiply, and cause disease. A compromised immune system does not just mean HIV, but includes periods when your body is dealing with the common cold, changes in diet, stress, or chemotherapy. If you have any reason to be concerned about taking this course, please talk with your doctor prior to enrolling in BIo103L and participating in the laboratory procedures.

If you do not have a compromised immune system and are concerned for minor reasons (such as cuts, scrapes, or a cold), be cautious and wear gloves in lab. No one will fault you for being careful. Even benign microbes may evolve pathogenicity, so treat all microorganisms as potential pathogens and follow lab safety protocols. The microbiology staff does!

Extra Hours Required: Late in the semester, you are required to attend several extra hour sessions where you work on your own to set up tests to identify 2 unknown microorganisms. TAs are on duty during these extra hour sessions to answer questions and help guide you through the process. These unknown experiments take a lot of time outside lab but are very rewardinig. Example extra hour schedules available on Blackboard.
Text and laboratory manual: (available at the Duke University Bookstore) 

Brock Biology of Microorganisms.   Madigan, M. T. and J. Parker.   2006.   11th edition.   Pearson Education, Inc., Upper Saddle River, NJ.

Laboratory Exercises for Bio 103 General Microbiology.   Golonka, A. M. and R. Vilgalys.   2006.   Spring Semester Issue.   Duke University, Department of Biology, Durham, NC.
Exams: There are two lecture exams and a final. Quizzes are given regularly during lab, and there is a lab practical at the end of the semester.

Exam format: Exam questions will be multiple choice and short answer, along with short essays. The extent of the material covered on exams will be announced in class. The syllabus shown is tentative and meant only as a guide. However, the exam dates are firm. Students are expected to conform to the Judicial Code and Honor Commitment regarding exams.

Make-up exam policy: Make-ups will usually be given within one week after the scheduled exam, at a time and place to be announced. Make-ups for reasons other than illness require notification in advance. Please do not call the instructor directly before an exam. It is your responsibility to insure that each exam is taken at the proper time.
Lecture Grade: 69% of course grade.
Based on:
Exam 1......................................23%
Exam 2......................................23%
Final...........................................23% 
Lab Grade: 31% of course grade
Based on:
Quizzes and homework...........................25%
Lab Report 1............................................15%
Two unknown reports...............................30%
Attendance and participation.................10%
Lab practical............................................20%
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